In a world that is growing more and more populated with cell lines, it can be difficult to establish the best model for each experiment. From animal models to human cell cultures, this article shows you how to use transgenic mice to build a cell line model.
What is a transgenic mouse?
A transgenic mouse is created when a gene from one species is introduced into another. This can be done by either inserting the gene into the mouse’s genome using traditional breeding methods or by using a viral vector to deliver the gene directly. Transgenic mice are valuable because they allow scientists to study the effects of a particular gene on an organism without having to experiment on humans.
What does it take to create cell line models in transgenic mice
To build a cell line model using transgenic mice, you will need the following supplies:
1) Transgenic mice (or other mice with added genes)
2) A breeding ground (e.g., culture dish)
3) A genetic engineering tool kit
4) Lab equipment (e.g., a DNA isolation kit, a centrifuge, a microscope, etc.)
5) Genomic DNA (from the transgenic mouse)
6) Polyethylene glycol 8000 (PEG 8000) or sterile water
7) Ethanol or isopropanol
8) Sodium hypochlorite solution (or bleach)
What are the benefits of using transgenic mice to create cell line models?
There are many benefits to using transgenic mice to create cell line models. First, transgenic mice are easy to work with; they have a predictable genetic background and can be bred quickly. Second, transgenic mice are accurate models of human disease; their cells carry the genetic mutations that cause diseases, and these mutations can be easily transferred to other cells in the mouse model. Finally, transgenic mice can be used to study the mechanisms underlying disease progression, and they can be used to test new treatments.
With the help of transgenic mice, we can build cell line models that closely resemble human cells and tissues. If you are interested in learning more about how to use transgenic mice to build cell line models, be sure to contact us!